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Thursday, July 30, 2020 | History

4 edition of Morphological investigations of single neurons in vitro found in the catalog.

Morphological investigations of single neurons in vitro

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Published by Wiley in Chichester, New York .
Written in English

    Subjects:
  • Neurons -- Ultrastructure.,
  • Neuroanatomy.,
  • Nerves -- Histology.

  • Edition Notes

    Includes bibliographical references and index.

    Statementedited by Gloria E. Meredith and Gordon W. Arbuthnott.
    SeriesIBRO handbook series ;, v. 16
    ContributionsMeredith, Gloria E., Arbuthnott, Gordon W.
    Classifications
    LC ClassificationsQM575 .M67 1993
    The Physical Object
    Paginationxvi, 193 p. :
    Number of Pages193
    ID Numbers
    Open LibraryOL1402313M
    ISBN 100471939285
    LC Control Number93010269

      In vivo two-photon calcium imaging provides detailed information about the activity and response properties of individual neurons. However, in vitro methods are often required to study the Cited by: 6. The fundamental morphology/cytology of the neuron has not been well represented in the literature. This is primarily due to the great variety in neural forms and their very high aspect ratio. There are a profusion of caricatures at the conceptual level but little precise information concerning the relevant detailed cytology of the cell.

      These neuronal losses include cholinergic neurons, noradrenergic neurons and dopaminergic neurons which play a critical role in brain function by releasing a neurotransmitter called dopam13 Cited by: 9.   3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function. P S Timashev 1, M V Vedunova 2, D Guseva 3, E Ponimaskin 3, A Deiwick 4, T A Mishchenko 2, E V Mitroshina 2, A V Koroleva 4, A S Pimashkin 2, I V Mukhina 2, V Ya Panchenko 1, B N Chichkov 1 and V N Bagratashvili 1Cited by:

    A cultured neuronal network is a cell culture of neurons that is used as a model to study the central nervous system, especially the , cultured neuronal networks are connected to an input/output device such as a multi-electrode array (MEA), thus allowing two-way communication between the researcher and the network. This model has proved to be an invaluable tool to scientists.   Scientists at Karolinska Institutet in Sweden have developed a new technique that enables them to isolate single neurons from tissues and analyse .


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Morphological investigations of single neurons in vitro Download PDF EPUB FB2

Morphological Investigations of Single Neurons in Vitro brings together a variety of applications for isolated preparations. Although in vitro preparations have long yielded valuable data in physiological and pharmacological studies, their anatomical power has only recently come to light.

Neuromorphology (from Greek νεῦρον, neuron, "nerve"; μορφή, morphé, "form"; -λογία, -logia, “study of”) is the study of nervous system form, shape, and structure.

The study involves looking at a particular part of the nervous system from a molecular and cellular level and connecting it to a physiological and anatomical point of view. Following plating in vitro, neurons pass through a series of morphological stages as they adhere and mature.

These morphological stage transitions can be monitored as a function of time to evaluate the relative health and development of neuronal cultures under different by: Neurophysiological, pharmacological and morphological properties of caudate neurons were characterized by intracellular recordings in an in vitro slice preparation.

These properties were compared with those of tissue obtained from animal studies. Electrophysiological properties of human caudate neurons that were similar to those of cat caudate Cited by: We use in vitro neuronal networks as a model system for studying self-organization processes in the nervous system.

We follow the neuronal growth process, from isolated neurons to fully connected two-dimensional networks. The mature networks are mapped into connected graphs and their morphological characteristics are measured.

The first differentiation of enteric neurons into three morphological types was done by the russian histologist A. Dogiel on the basis of the different shapes and lengths of their dendrites.

Although a number of authors considered his results during the following decades, only a division into two types withstood time: type I neurons had one long and several short processes, whereas type II Cited by: 1.

Author(s): Meredith,Gloria E; Arbuthnott,Gordon W Title(s): Morphological investigations of single neurons in vitro/ edited by Gloria E. Meredith and Gordon W. Arbuthnott. This work was aimed at the morphological and biochemical characterisation of the most susceptible neuronal subpopulation to rabies virus (RABV) infection.

Adult mouse DRG cultures were infected with RABV and double-processed for viral antigen detection and neuropeptides: calcitonine gene-related peptide (CGRP), galanin (GAL), substance P (SP), neuropeptide Y (NPY) and Cited by: 8. Book review Full text access IBRO handbook series: Methods in the neurosciences.

volume Morphological investigations of single neurons in vitro. Edited by G. Meredith and G. Arbuthnott. Wiley, Chichester, U.K. Price £ 1 An in vitro model for neuroscience - differentiation of SH-SY5Y cells into cells with morphological and biochemical characteristics of mature neurons Running title: A novel in vitro model for neuroscience Lotta Agholme*, Tobias Lindström1, 2, Katarina Kågedal3, Jan Marcusson1, and Martin Hallbeck2 Department of Clinical and Experimental Medicine, 1.

Juxtacellular recording and morphological identification of single neurons in freely moving rats Article in Nature Protocols 9(10) October with Reads How we measure 'reads'. Brain functions are strictly dependent on neural connections formed during development and modified during life.

The cellular and molecular mechanisms underlying synaptogenesis and plastic changes involved in learning and memory have been analyzed in detail in simple animals such as invertebrates and in circuits of mammalian brains mainly by intracellular recordings of neuronal by: Labeling neurons in vivo for morphological and functional studies Article Literature Review in Current Opinion in Neurobiology 14(5) November with 72 Reads How we measure 'reads'.

Distinctive Morphological Features of a Subset of Cortical Neurons Grown in the Presence of Basal Forebrain Neurons In Vitro Dun H. Ha, Richard T. Robertson, John H. Weiss Journal of Neuroscience 1 June18 (11) ; DOI: /JNEUROSCICited by: Neuronal Activity Patterns During Hippocampal Network Oscillations In Vitro Fig.

2 Properties of distal (O-LM) and proximal (trilaminar) dendrite-targeting interneurons. A Morphology of O-LM and trilaminar cells. Somata and dendrites are drawn in red, axons are in somata of O-LM cells are located in stratum oriens and have mainly horizontally run.

Detailed analysis of dendritic spine morphology and motility is an essential tool for investigating the potential effects of mutant synaptic proteins, and how mutations in these disease-associated synaptic proteins may affect synapse structure and function, thereby contributing to the pathophysiology of a number of disorders of the by: in vitro assays that monitor neuronal function.

We hypothesized that (1) toxic insults to the nervous system will cause neuronal synapses to deteriorate in the early phase of neurotoxicity, eventually leading to neurite degeneration and neuronal cell death if the damage is severe; and (2) an. transgenic lines in which small subsets of neurons are labeled brightly [17].

Of particular interest are the few lines that express XFP in isolated single neurons in accessible regions of the nervous system such as the neocortex, olfactory bulb and the neuromuscular junction (Figure 1).

The combination of. Our aim was to develop an improved in vitro model, generating sustainable cells with morphology and biochemistry of human, mature neurons. To obtain cells with neuronal differentiation and function, we investigated the effect of combining three-dimensional culturing of SH-SY5Y cells in extracellular matrix (ECM) gel with several factors Cited by: [57] Houser C R, Crawford G D, Barber R P, Salvaterra P M, Vaughn J E.

Organization and morphological characteristics of cholinergic neurons: an immunocytochemical study with a monoclonal antibody to choline acetyltransferase.

Quantitative assessments of neuronal subtypes in numerous brain regions show large variations in dendritic arbor size. A critical experimental factor is the method used to visualize neurons. We chose to investigate quantitative differences in basolateral amygdala (BLA) principal neuron morphology using two of the most common visualization methods: Golgi–Cox staining and neurobiotin (NB) by: 6.In vitro systems, however, Investigations into anatoxin-a, This neurite growth inhibition can often lead to defects in neural migration, and significant morphological changes of neurons during development,) often leading to neural tube defects in neonates.ORIGINAL RESEARCH Recording, labeling, and transfection of single neurons in deep brain structures Bowen Dempsey1, Anita J.

Turner1, Sheng Le1, Qi-Jian Sun1, Lama Bou Farah1, Andrew M. Allen2, Ann K. Goodchild1 & Simon McMullan1 1 Australian School of Advanced Medicine, Macquarie University, Sydney, NSW,AustraliaCited by: